王华磊
  • 单位：华东理工大学   生物工程学院
  • 职称：副教授
  • 职位：无
  • 出生：1983.02
  • 性别：男
  • 社会职务：
  • 联系方式：13681859527;hlwang@ecust.edu.cn
  • 微博：

研究领域

（1）以酶资源多样性、酶催化机理、理性设计方法学为研究内容的生物催化剂高效创制。

（2）以“高值化”产品为导向、基于逆合成生物催化技术的手性药物中间体绿色高效合成工艺开发。

论文与著作

  1. 1.Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Qinghai Liu, Yuhong Ren, Dongzhi Wei. Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles. Biotechnology Letters, 2017, 39(11): 1725–1731.

  2. 2.Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Qinghai Liu, Yuhong Ren, Dongzhi Wei. Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile. Chemical Papers, 2017, 71(10): 1985–1992.

  3. 3.Haiyang Fan, Lifeng Chen, Huihui Sun, Hualei Wang*, Yuhong Ren, Dongzhi Wei. A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production. Bioprocess and Biosystems Engineering, 2017, 40(8):1271-1281.

  4. 4.Lifeng Chen, Haiyang Fan, Yiping Zhang, Wei Wei, Jinping Lin, Dongzhi Wei, Hualei Wang*. Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption. Journal of biotechnology, 2017, 251:68-75.

  5. 5.Lifeng Chen, Yiping Zhang, Haiyang Fan, Kai Wu, Jinping Lin, Hualei Wang*, Dongzhi Wei. Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase. Catalysis Communications, 2017, 97:5-9.

  6. 6.Lifeng Chen, Haiyang Fan, Yiping Zhang, Kai Wu, Hualei Wang*, Jinping Lin, Dongzhi Wei. Development of a practical biocatalytic process for (S)-N-Boc-3-hydroxypiperidine synthesis. Tetrahedron Letters, 2017, 58(16):1644–1650.

  7. 7.Yiping Zhang, Hualei Wang*, Lifeng Chen, Kai Wu, Jingli Xie, Dongzhi Wei. Efficient production of ethyl (R)-4-chloro-3-hydroxybutanoate by a novel alcohol dehydrogenase from Lactobacillus curieae S1L19.Journal of Molecular Catalysis B: Enzymatic. 2016, 134:51-60

  8. 8.Wenyuan Gao, Haiyang Fan, Lifeng Chen, Hualei Wang*, Dongzhi Wei. Efficient kinetic resolution of secondary alcohols using an organic solvent-tolerant esterase in non-aqueous medium. Biotechnology Letters. 2016,38(7):1165-1171

  9. 9.Kai Wu, Hualei Wang#*, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Dongzhi Wei*. Practical two-step synthesis of enantiopure styrene oxide through an optimized chemoenzymatic approach.Applied Microbiology and Biotechnology.2016, 100(20):8757-8767

  10. 10.Zhiqiang Zhao, Hualei Wang*, Yiping Zhang, Lifeng Chen, Kai Wu, Dongzhi Wei. Cloning and characterization of three ketoreductases from soil metagenome forpreparing optically active alcohols. Biotechnology Letters.2016, 38(10):1799-1808

  11. 11.Wenyuan Gao, Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Bei Gao, Hualei Wang*, Dongzhi Wei*. A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters. Microbial Cell Factories, 2016, 15:41.

  12. 12.Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Hualei Wang*, Dongzhi Wei*. Synthesis of enantiopure epoxide by ‘one pot’ chemoenzymatic approach using a highly enantioselective dehydrogenase. Tetrahedron Letters, 2016, 57(8):899-904.

  13. 13.Huihui Sun, Wenyuan Gao, Hualei Wang*, Dongzhi Wei. Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S-selectivity toward mandelonitrile present in active inclusion bodies. Biotechnology letters, 2016，38(3):455-461

  14. 14.Hualei Wang, Haiyang Fan, Huihui Sun, Li Zhao, Dongzhi Wei*. Process development for production of (R)-(-)-mandelic acid by recombinant Escherichia coli cells harboring nitrilase from Burkholderia cenocepacia J2315. Organic Process Research & Development, 2015,19(12),2010-2016

  15. 15.Hualei Wang, Wenyuan Gao, Huihui Sun, Lifeng Chen, Lujia Zhang, Xuedong Wang*, Dongzhi Wei*. Protein engineering of nitrilase from Burkholderia cenocepacia J2315 for efficient and enantioselective production of (R)-o-chloromandelic acid. Applied and Environmental Microbiology, 2015, 81(24):8469-8477.

  16. 16.Huihui Sun, Hualei Wang*, Wenyuan Gao, Lifeng Chen, Kai Wu, Dongzhi Wei*. Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile. Biochemical and Biophysical Research Communications, 2015, 468(4):820-825.

  17. 17.Huihui Sun, Wenyuan Gao, Haiyang Fan, Hualei Wang*, Dongzhi Wei. Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives. Biotechnology letters, 2015，37(8):1655–1661.

  18. 18.Kai Wu, Hualei Wang*,Huihui Sun,Dongzhi Wei*. Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurella paurometabola. Applied Microbiology and Biotechnology, 2015, 99(22):9511-9521

  19. 19.Hualei Wang, Huihui Sun, Wenyuan Gao, Dongzhi Wei*. Efficient production of (R)-o-chloromandelic acid by recombinant Escherichia colicells harboring nitrilase from Burkholderia cenocepacia J2315. Organic Process Research & Development, 2014, 18(6):767–773.

  20. 20.Hualei Wang, Guinan Li, Mingyang Li, Dongzhi Wei*, Xuedong Wang*. A novel nitrilase from Rhodobacter sphaeroides LHS-305-cloning, heterologous expression and biochemical characterization. World Journal of Microbiology and Biotechnology, 2014, 30(1):245-252.

  21. 21.Hualei Wang,Huihui Sun, Dongzhi Wei*. Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction．BMC Biotechnology, 2013, 13:14.

  22. 22.Kefeng Ni, Hualei Wang（并列第一作者）, Li Zhao, Minjie Zhang, Siyuan Zhang, Yuhong Ren*, Dongzhi Wei*. Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E.coli.Journal of Biotechnology, 2013, 167(4):433-440.

  23. 23.Shuiqin Jiang, Lujia Zhang*, Zhiqiang Yao, Bei Gao, Hualei Wang, Xiangzhao Mao, Dongzhi Wei*. Switching a nitrilase from Syechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science & Technology, 2017, 7, 1122-1128.

  24. 24.Jian Qiu, Erzheng Su*, Hualei Wang, Wenwen Cai, Wei Wang, Dongzhi Wei*. Cloning, overexpression, and characterization of a high enantioselective nitrilase from Sphingomonas wittichii RW1 for asymmetric synthesis of (R)-phenylglycine. Applied Biochemistry and Biotechnology, 2014, 173(2):365-377.

  25. 25.Lujia Zhang, Bo Yin, Chao Wang, Shuiqin Jiang, Hualei Wang, Dongzhi Wei*, Y Adam Yuan*. Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp. PCC6803.Journal of Structural Biology, 2014, 188(2):93-101.

  26. 26.KefengNi, XuZhou, LiZhao,HualeiWang, YuhongRen, DongzhiWei*. Magnetic Catechol-Chitosan with Bioinspired Adhesive Surface: Preparation and Immobilization of omega-Transaminase. Plos One, 2012, 7(7):e41101

专利：

1、魏东芝、许向阳、宋在伟、王华磊。一种生物法制备苯乙酸的方法。中国发明专利，申请号：201611110169.8。

2、魏东芝、许向阳、宋在伟、王华磊。一种生物法合成(R)‑邻氯扁桃酸的方法。中国发明专利，申请号：201611110170.0。

3、王华磊、谢有余、王继国、范海洋、魏东芝。一种S型的ω-转氨酶ATA-W12及其基因和应用，中国发明专利，申请号：201611055835.2。

4、王华磊、张一平、魏东芝。一种醇脱氢酶LC3及其基因和应用，中国发明专利，申请号：201610066375.7。

5、王华磊、范海洋、任宇红、魏东芝。一种生物法合成(R)-(-)-扁桃酸的方法，中国发明专利，申请号：201510334809.2。

6、王华磊、高文渊、魏东芝。酯酶est4、酯酶EST4、重组质粒和基因工程菌株及其应用，中国发明专利，授权号：ZL 201510342723.4。

7、王华磊、陈利锋、魏东芝。一种不对称转化制备(S)-4-氯-3-羟基丁酸乙酯的重组大肠杆菌及其应用，中国发明专利，申请号：201510098687.1。

8、王华磊、吴锴、魏东芝。一种环氧化物水解酶及其编码基因和应用，中国发明专利，授权号：ZL 201410175055.8。

荣誉与奖励

2010年11月参与完成的“工业酶制剂的创制与应用关键技术”获上海市科技进步一等奖；2013年12月参与完成的“定向转化多元醇的细胞催化剂创制及其应用关键技术”获上海市技术发明一等奖。